Speaker
Dr
Jasenko Zivanov
Description
Three new computational methods will be presented that aim to improve the maximal attainable resolution of single-particle cryo-EM reconstructions by measuring and correcting for optical effects in the microscope. All methods work on a given data set, so they can be applied after the images have been collected. The first two methods allow us to measure the symmetrical and antisymmetrical higher-order optical aberrations (which deform the CTF and induce a phase shift, respectively), while the third method measures the magnification anisotropy. The methods are computationally efficient, and implementations will be provided with the upcoming release of Relion.
