NYC Computational Cryo-EM Summer Workshop

Session

Chair: Carlos Oscar Sorzano

Aug 8, 2019, 11:00 AM

Ingrid Daubechies Auditorium (Flatiron Institute)

11. Ayelet Heimowitz; Automatic particle picking and centering

Dr Ayelet Heimowitz

8/8/19, 11:00 AM

"Particle picking is a crucial first step in the computational pipeline of single-particle cryo-electron microscopy. Selecting particles from the micrographs is difficult especially for small particles with low contrast. As high-resolution reconstruction typically requires hundreds of thousands of particles, including rare views, manual picking is often too time-consuming. While template-based...

7. Joe Kileel; Autocorrelation analysis for cryo-EM and XFEL

Dr Joe Kileel

8/8/19, 11:40 AM

In 1980, Zvi Kam introduced an autocorrelation-based approach to cryo-electron microscopy (cryo-EM) single particle reconstruction, in which moments of the 2D images are computed and the 3D molecule is recovered by solving a polynomial system of equations. Recently, the method has also been used in X-ray free electron laser (XFEL) imaging.

This talk addresses important challenges in applying...