NYC Computational Cryo-EM Summer Workshop

Session

Chair: Marina Spivak

Aug 9, 2019, 3:50 PM

Ingrid Daubechies Auditorium (Flatiron Institute)

20. Ali Punjani; Cryo-EM Resolution Estimation

Dr Ali Punjani

8/9/19, 3:50 PM

Resolution estimation is a critical and practical aspect of cryo-EM reconstruction. In this project, we explore a new way to think about resolution estimation in cryo-EM. We apply the principle of cross-validation as a general idea to develop a framework, from which tasks like resolution estimation can be formulated, and solutions can be derived from first principles. The resulting solutions...

21. Gabor Herman; The Efficacy of Iterative Algorithms for Reconstruction from Cryo-EM Data

Dr Gabor Herman

8/9/19, 4:30 PM

• Tomographic data collection methods (of which cryo-EM is an example) provide us with approximations of line integrals of some parameter.
• Reconstruction is the attempted recovery of the distribution of the parameter values from such data.
• The two major categories of reconstruction techniques are:
– transform methods such as weighted backprojection (WBP) and FBP; and
– series expansion...