Resolution estimation is a critical and practical aspect of cryo-EM reconstruction. In this project, we explore a new way to think about resolution estimation in cryo-EM. We apply the principle of cross-validation as a general idea to develop a framework, from which tasks like resolution estimation can be formulated, and solutions can be derived from first principles. The resulting solutions...
• Tomographic data collection methods (of which cryo-EM is an example) provide us with approximations of line integrals of some parameter. • Reconstruction is the attempted recovery of the distribution of the parameter values from such data. • The two major categories of reconstruction techniques are: – transform methods such as weighted backprojection (WBP) and FBP; and – series expansion...