June 11, 2020
Virtual
America/New_York timezone

Synapse-level Connectomics: Reconstruction of a Mini-insect

Jun 11, 2020, 12:00 PM
30m
Dmitri Chklovskii, Ph.D. (Group Leader, Neuroscience, Center for Computational Biology, Flatiron Institute)

Dmitri Chklovskii, Ph.D.

Group Leader, Neuroscience, Center for Computational Biology, Flatiron Institute

Description

Maps of synaptic connections between neurons, or connectomes, provide crucial information for reverse
engineering the brain. We developed a semi-automated image processing pipeline to reconstruct the connectome from serial electron micrographs. We report first results from the connectome of Megaphragma amalphitanum, a microscopic wasp whose linear size is an order of magnitude smaller than that of Drosophila. One peculiar feature of the Megaphragma is that most neurons lack nuclei. We find that the few neurons with nuclei occupy stereotypical positions in the connectome suggesting that their function requires genetic information.

Joint work with the groups of Alexei Polilov (Moscow),
Harald Hess (Janelia), and Viren Jain (Google).

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