Presenter: Rahul Satija, Ph.D., New York Genome Center
Integrative analysis of multi-modal single-cell data
Single-cell RNA-seq has proven to be a transformative tool for the discovery of cell types and states, but the transcriptome reflects only one aspect of cellular identity. The next frontier for single-cell genomics is 'multi-modal' analysis, where multiple types of measurements are simultaneously collected from individual cells, often in parallel with gene expression. For example, the CITE-seq technology allows for RNA and protein levels to be simultaneously measured in single cells.
In this talk, I will introduce new statistical methods designed for ‘multi-view’ learning based on multi-modal data. We introduce an unsupervised strategy to quantify the information content related to each modality in each cell, and construct a weighted kernel that integrates information from each source. We apply this approach to a CITE-seq dataset of 250,000 human blood cells with 220 surface proteins, and construct an integrated atlas of the human immune system, and demonstrated. This atlas can be used to map and interpret additional datasets of immune perturbations, for example, to identify specific immune cell types that are affected during response to vaccination, sepsis, or COVID-19.
Rahul Satija, Ph.D., is a Core Faculty Member at the New York Genome Center (NYGC), with a joint appointment as Associate Professor at the Center for Genomics and Systems Biology at New York University (NYU). Prior to joining the NYGC, Dr. Satija was a postdoctoral researcher at the Broad Institute of Harvard and MIT, where he developed new methods for single-cell analysis.
The Satija Lab focuses on developing computational and experimental methods to sequence and interpret the molecular contents of a single cell. His Lab applies single-cell genomics to understand the causes and consequences of cell-to-cell variation, with a particular focus on immune regulation and early development.
The National Institutes of Health selected Dr. Satija for the prestigious New Innovator Award to fund research that focuses on how the interaction between molecular and environmental factors governs cell behavior.
Dr. Satija holds a BS degree in Biology and Music from Duke University and obtained his Ph.D. in Statistics from Oxford University as a Rhodes Scholar.
